Sentences with LYSATE
Check out our example sentences below to help you understand the context.Sentences
1
"The researchers obtained a cell lysate from the culture to use in their experiments."
2
"The lysate solution was then centrifuged to separate the cellular debris."
3
"They measured the protein concentration in the lysate using a spectrophotometer."
4
"The lysate was diluted before being added to the reaction mixture."
5
"The enzymatic activity of the lysate was assessed using a colorimetric assay."
6
"The lysate was treated with a protease inhibitor to prevent protein degradation."
7
"They used a freeze-thaw cycle to disrupt the cells and release the lysate."
8
"The researchers added the lysate to the antibody-coated plate for immunoprecipitation."
9
"The expression of the target protein was analyzed by western blotting of the lysate."
10
"After sonication, the lysate was cleared by high-speed centrifugation."
11
"The lysate was stored at -80°C until further analysis."
12
"The activity of the lysate was compared to a positive control."
13
"They prepared a fresh lysate for each experimental replicate."
14
"The lysate was filtered through a 0.22 μm membrane to remove any particulates."
15
"The concentration of a specific metabolite in the lysate was measured."
16
"They added a reducing agent to the lysate to break disulfide bonds."
17
"The lysate was incubated with magnetic beads for affinity purification."
18
"The researchers observed a band of the target protein in the lysate gel."
19
"The lysate was gently mixed to ensure homogeneity before use."
20
"They used a detergent to solubilize membrane proteins in the lysate."
1
"The lysate was obtained after cell lysis."
2
"The lysate was centrifuged to separate the cellular debris."
3
"The protein concentration in the lysate was measured using a spectrophotometer."
4
"The lysate was loaded onto a gel for protein separation."
5
"The lysate was diluted before being used in the assay."
6
"The lysate was incubated with the substrate for enzyme activity measurement."
7
"The lysate contained high levels of recombinant protein."
8
"The lysate was treated with a specific antibody for immunoprecipitation."
9
"The addition of protease inhibitors ensured the stability of the lysate."
10
"The lysate was stored at -80 degrees Celsius for long-term preservation."
